| Identification |
|---|
| BMDB Protein ID
| BMDBP00459 |
| Secondary Accession Numbers
| None |
| Name
| Myosin light chain kinase, smooth muscle |
| Synonyms
|
Not Available
|
| Gene Name
| MYLK |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Involved in actin binding |
| Specific Function
| Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells (By similarity). |
| Pathways
|
- Intracellular Signalling Through Prostacyclin Receptor and Prostacyclin
- Muscle/Heart Contraction
- Rac 1 cell motility signaling pathway
- Rac 1 Cell Motility Signaling Pathway
|
| Reactions
|
| Myosin light chain 3 → Myosin light chain 3 + Hydrogen phosphate |
details
|
|
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 1 |
| Locus
| Not Available |
| SNPs
| MYLK |
| Gene Sequence
|
>3531 bp
ATGGATTTCCGCGCCAACCTGCAGCGGCAGGTGAAGCCGAAGACCCTGTCAGAGGAGGAG
AGGAAAGTGCATGGCCCCCAGCAGGTGGACTTCCGCTCTGTCCTAGCCAAGAAGGGGACT
CCCAAGACCCCTGTGCCTGAGAAGGTACCCCCTCCAAAACCGGCCACGCCAGATTTTCGC
TCAGTGTTGGGCAGTAAGAAAAAATTACCAACAGAGAATGGTAGCAATAACACTGAGGCC
TTGAATGCCAAGGCAGCAGAAGGTCTCAAGCCTGTGGGCAATGCCCAGCCTTCAGGGTTC
CTGAAACCCGTGGGCAACGCCAAGCTGGCTGATACCCCCAAACCCTTGAGCAGCACCAAG
CCAGCAGAGACCCCCAAACCCTTGGGTAATGTCAAGCCAGCTGAGACCCCCAAACCCTTG
GGCAGCACCAAGCCAGCAGAAACCCCCAAACCCTTGGGCAGCACCAAGCCAGCAGAGACC
CCAAAACCCTTGGGTAACGTCAAGCCAGCTGAGACCCCCAAACCCTTGGGTAACATCAAG
CCAACTGAGACCCCCAAACCCTTGGGCAGCACCAAGCCAGCAGAAACCCCCAAACCCTTG
GGCAGCACCAAGCCAGCAGAGACCCCAAAACCCTTGGGTAATGTCAAGCCAGCCGAGACC
CCCAAACCCTTGGGTAATGTCAAGCCAGCTGAGACCCCCAAACCCTTGGGTAATGTCAAG
CCAGCTGAGACCCCTAAACCTGTGAGCAATGCCAAGCCAGCCGAAACCCTCAAACCTGTG
GGCAACGCCAAGCCAGCTGAGACCCCCAAACCCTTGAGTAACGTCAAGCCAGCTGAAACC
CCCAAACTTGTGGGCAATGCCAAGCCAGCTGAGACCTCTAAACCCTTGGATAACGCCAAG
CCAGCTGAGGCCCCCAAACCCTTGGGCAATGCCAAGCCGGCTGAAATCCCAAAACCCACT
GGGAAAGAAGAACTTAAGAAGGAGATTAAGAATGATGTGAACTGCAAGAAGGGCCATGCA
GGGGCCACAGATAGTGAAAAGAGACCAGAGAGCCGAGGGACAGCCCCGACCTTCGAGGAG
AAACTACAAGATCTCCATGTGGCAGAGGGTCAGAAGCTGCTGCTCCAGTGCCGGGTGTCC
TCTGACCCCCCAGCCACCATCACTTGGACACTGAATGGAAAGACGCTCAAGACCACCAAG
TTCATCGTCCTCTCCCAAGAAGGCTCCTTGTGCTCCGTCTCCATCGAGAAGGCACTGCCC
GAGGACAGAGGCTTATACAAGTGTGTAGCCAAGAACAGTGCCGGCCAGGCTGAGTCCTCC
TGCCAAGTCACCGTGGACGTTCCAGATGCCCCCACCAGTGAAAATGCCAAGGCCCCGGAG
ATGAAAGCCCGGAGGCCGAAGAGCTCTCTTCCTCCAGTGCTAGGAACAGAAAGTGACGCA
ACTGTGAAAAAGAAACCTGCCCCCAAGACACCTCCGAAGGCAGCCATGCCGCCTCAGATC
ATCCAGTTTCCTGAGGACCAGAAGGTGCGAGCAGGAGAGTCTGTGGAGCTCTTTGGTAAG
GTGGCCGGCACCCAGCCCATCACCTGCACCTGGATGAAGTTCCGAAAGCAGATCCAGGAC
AGCGAGCACATCAAGGTGGAGAACAGCGAGCAGGGCAGCAAGCTCACCATCCGGGCGGCA
CGCCAGGAGCACTGTGGCTGCTACACCCTGCTGGTGGAGAACAAACTGGGCAGCAGGCAG
GCCCAAGTCAACCTCACCGTGGTGGACAAGCCAGACCCCCCAGCCGGTACACCTTGTGCC
TCTGACATCCGAAGCTCCTCACTGACCTTGTCCTGGTATGGCTCCTCGTATGATGGGGGC
AGCGCCGTACAGTCATACAGTGTCGAGATCTGGGACTCAGTGGACAAGACGTGGAAGGAA
CTAGCCACGTGCCGCAGCACTTCTTTCAACGTCCAGGACCTGCTGCCTGACCGAGAATAT
AAGTTCCGTGTGCGTGCAATCAATGTCTATGGAACCAGTGAGCCAAGCCAGGAGTCTGAA
CTCACAGCCTTGGGAGAGAAACCTGAAGAAGAGCCGAAGGACGAAGTGGAGGTGTCAGAT
GATGATGAGAAGGAGCCTGAGGTCGACTACCGGACCGTCACGGTCAATACCGAGCAGAAA
GTGTCCGACTTCTACGACATCGAAGAGAGACTAGGATCTGGGAAATTTGGACAGGTCTTT
CGACTTGTAGAAAAGAAAACTGGAAAAATCTGGGCAGGGAAATTCTTCAAGGCGTATTCG
GCCAAGGAGAAAGAGAACATCCGGCAGGAGATCAGCATCATGAACTGCCTGCACCACCCC
AAGCTGGTCCAGTGTGTGGACGCCTTCGAAGAGAAGGCCAACATCGTCATGGTCCTGGAG
ATCGTGTCCGGGGGTGAGCTGTTCGAGCGCATCATTGATGAGGACTTTGAGCTGACGGAG
CGGGAGTGCATCAAGTACATGAAGCAGATCTCAGAGGGGGTGGAGTACATCCACAAGCAG
GGCATCGTCCACCTGGACCTCAAGCCCGAGAACATCATGTGTGTCAACAAGACAGGCACC
AGGATCAAGCTCATCGACTTCGGTCTGGCCAGGAGGTTGGAGAATGCGGGGTCTCTGAAG
GTCCTCTTTGGCACCCCGGAGTTCGTCGCACCCGAAGTGATCAACTATGAGCCCATTGGC
TATGCCACGGACATGTGGAGCATCGGGGTCATCTGCTACATCCTGGTCAGTGGACTGTCC
CCCTTCATGGGTGACAATGACAACGAAACCTTAGCCAACGTTACCTCAGCCACCTGGGAC
TTTGACGACGAGGCCTTCGACGAGATCTCCGATGACGCCAAGGATTTTATCAGCAACTTG
CTGAAGAAGGATATGAAAAACCGCCTGAACTGCACCCAGTGCCTTCAGCATCCATGGCTA
ATGAAAGACACCAAGAACATGGAGGCCAAGAAACTCTCCAAGGACCGGATGAAGAAGTAC
ATGGCAAGAAGGAAATGGCAGAAAACGGGCAATGCTGTGAGAGCCATCGGAAGACTGTCC
TCTATGGCAATGATCTCAGGGCTCAGTGGCAGGAAATCCTCAACAGGGTCACCAACCAGC
CCGCTCAATGCAGAAAAACTAGAATCTGAAGATGTCTCCCAAGCTTTCCTGGAGGCTGTC
GCTGAGGAGAAGCCCCATGTAAAACCCTACTTTTCCAAGACCATTCGTGACTTAGAAGTT
GTGGAGGGAAGTGCTGCTAGATTTGACTGCAAGATTGAAGGATACCCGGACCCTGAAGTC
GTCTGGTTCAAAGATGACCAATCAATCCGGGAGTCCCGCCACTTCCAGATAGACTACGAT
GAGGATGGGAACTGCTCTTTAATCATTAGTGATGTCTGCGGGGATGATGATGCCAAGTAC
ACCTGCAAGGCTGTCAACAGTCTTGGAGAAGCCACCTGCACAGCAGAGCTCATCGTGGAA
ACCATGGAGGAAGGAGAAGGAGAAGGGGGAGAAGAGGAGGAGGAAGAGTGA
|
| Protein Properties |
|---|
| Number of Residues
| 1176 |
| Molecular Weight
| 128825.0 |
| Theoretical pI
| 6.43 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
- 66-86
- 90-110
- 127-147
- 154-174
- 184-204
- 216-236
- 613-633
- 637-657
- 676-696
- 727-747
- 755-775
- 777-797
|
| Protein Sequence
|
>Myosin light chain kinase, smooth muscle
MDFRANLQRQVKPKTLSEEERKVHGPQQVDFRSVLAKKGTPKTPVPEKVPPPKPATPDFR
SVLGSKKKLPTENGSNNTEALNAKAAEGLKPVGNAQPSGFLKPVGNAKLADTPKPLSSTK
PAETPKPLGNVKPAETPKPLGSTKPAETPKPLGSTKPAETPKPLGNVKPAETPKPLGNIK
PTETPKPLGSTKPAETPKPLGSTKPAETPKPLGNVKPAETPKPLGNVKPAETPKPLGNVK
PAETPKPVSNAKPAETLKPVGNAKPAETPKPLSNVKPAETPKLVGNAKPAETSKPLDNAK
PAEAPKPLGNAKPAEIPKPTGKEELKKEIKNDVNCKKGHAGATDSEKRPESRGTAPTFEE
KLQDLHVAEGQKLLLQCRVSSDPPATITWTLNGKTLKTTKFIVLSQEGSLCSVSIEKALP
EDRGLYKCVAKNSAGQAESSCQVTVDVPDAPTSENAKAPEMKARRPKSSLPPVLGTESDA
TVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVAGTQPITCTWMKFRKQIQD
SEHIKVENSEQGSKLTIRAARQEHCGCYTLLVENKLGSRQAQVNLTVVDKPDPPAGTPCA
SDIRSSSLTLSWYGSSYDGGSAVQSYSVEIWDSVDKTWKELATCRSTSFNVQDLLPDREY
KFRVRAINVYGTSEPSQESELTALGEKPEEEPKDEVEVSDDDEKEPEVDYRTVTVNTEQK
VSDFYDIEERLGSGKFGQVFRLVEKKTGKIWAGKFFKAYSAKEKENIRQEISIMNCLHHP
KLVQCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMKQISEGVEYIHKQ
GIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLFGTPEFVAPEVINYEPIG
YATDMWSIGVICYILVSGLSPFMGDNDNETLANVTSATWDFDDEAFDEISDDAKDFISNL
LKKDMKNRLNCTQCLQHPWLMKDTKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLS
SMAMISGLSGRKSSTGSPTSPLNAEKLESEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEV
VEGSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDVCGDDDAKY
TCKAVNSLGEATCTAELIVETMEEGEGEGGEEEEEE
|
| External Links |
|---|
| GenBank ID Protein
| AAB25794.1 |
| UniProtKB/Swiss-Prot ID
| Q28824 |
| UniProtKB/Swiss-Prot Entry Name
| MYLK_BOVIN |
| PDB IDs
|
Not Available |
| GenBank Gene ID
| S57131 |
| GeneCard ID
| MYLK |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
