| Identification |
|---|
| BMDB Protein ID
| BMDBP00154 |
| Secondary Accession Numbers
| |
| Name
| Glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms
|
Not Available
|
| Gene Name
| GAPDH |
| Protein Type
| Enzyme |
| Biological Properties |
|---|
| General Function
| Carbohydrate transport and metabolism |
| Specific Function
| Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity). |
| Pathways
|
- Carbohydrate degradation
- Glycolysis
- Pyruvate from D-glyceraldehyde 3-phosphate: step 1/5
|
| Reactions
|
| D-Glyceraldehyde 3-phosphate + NAD + Hydrogen phosphate → Glyceric acid 1,3-biphosphate + NADH |
details
|
| D-Glyceraldehyde 3-phosphate + NAD → Glyceric acid 1,3-biphosphate + Hydrogen phosphate + NADH |
details
|
|
| GO Classification
|
Not Available
|
| Cellular Location
|
Not Available
|
| Gene Properties |
|---|
| Chromosome Location
| 5 |
| Locus
| Not Available |
| SNPs
| GAPDH |
| Gene Sequence
|
>1002 bp
ATGGTGAAGGTCGGAGTGAACGGATTCGGCCGCATCGGGCGCCTGGTCACCAGGGCTGCT
TTTAATTCTGGCAAAGTGGACATCGTCGCCATCAATGACCCCTTCATTGACCTTCACTAC
ATGGTCTACATGTTCCAGTATGATTCCACCCACGGCAAGTTCAACGGCACAGTCAAGGCA
GAGAACGGGAAGCTCGTCATCAATGGAAAGGCCATCACCATCTTCCAGGAGCGAGATCCT
GCCAACATCAAGTGGGGTGATGCTGGTGCTGAGTATGTGGTGGAGTCCACTGGGGTCTTC
ACTACCATGGAGAAGGCTGGGGCTCACTTGAAGGGTGGCGCCAAGAGGGTCATCATCTCT
GCACCTTCTGCCGATGCCCCCATGTTTGTGATGGGCGTGAACCACGAGAAGTATAACAAC
ACCCTCAAGATTGTCAGCAATGCCTCCTGCACCACCAACTGCTTGGCCCCCCTGGCCAAG
GTCATCCATGACCACTTTGGCATCGTGGAGGGACTTATGACCACTGTCCACGCCATCACT
GCCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAGCTGTGGCGTGACGGCCGAGGGGCT
GCCCAGAATATCATCCCTGCTTCTACTGGCGCTGCCAAGGCCGTGGGCAAGGTCATCCCT
GAGCTCAACGGGAAGCTCACTGGCATGGCCTTCCGCGTCCCCACTCCCAACGTGTCTGTT
GTGGATCTGACCTGCCGCCTGGAGAAACCTGCCAAGTATGATGAGATCAAGAAGGTGGTG
AAGCAGGCGTCAGAGGGCCCTCTCAAGGGCATTCTAGGCTACACTGAGGACCAGGTTGTC
TCCTGCGACTTCAACAGCGACACTCACTCTTCTACCTTCGATGCTGGGGCTGGCATTGCC
CTCAACGACCACTTTGTCAAGCTCATTTCCTGGTACGACAATGAATTTGGCTACAGCAAC
AGGGTGGTGGACCTCATGGTCCACATGGCCTCCAAGGAGTAA
|
| Protein Properties |
|---|
| Number of Residues
| 333 |
| Molecular Weight
| 35868.0 |
| Theoretical pI
| 8.58 |
| Pfam Domain Function
|
Not Available |
| Signals
|
|
|
Transmembrane Regions
|
Not Available
|
| Protein Sequence
|
>Glyceraldehyde-3-phosphate dehydrogenase
MVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLHYMVYMFQYDSTHGKFNGTVKA
ENGKLVINGKAITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIIS
APSADAPMFVMGVNHEKYNNTLKIVSNASCTTNCLAPLAKVIHDHFGIVEGLMTTVHAIT
ATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSV
VDLTCRLEKPAKYDEIKKVVKQASEGPLKGILGYTEDQVVSCDFNSDTHSSTFDAGAGIA
LNDHFVKLISWYDNEFGYSNRVVDLMVHMASKE
|
| External Links |
|---|
| GenBank ID Protein
| AAI02590.1 |
| UniProtKB/Swiss-Prot ID
| P10096 |
| UniProtKB/Swiss-Prot Entry Name
| G3P_BOVIN |
| PDB IDs
|
|
| GenBank Gene ID
| BC102589 |
| GeneCard ID
| GAPDH |
| GenAtlas ID
| Not Available |
| HGNC ID
| Not Available |
| References |
|---|
| General References
| Not Available |
